Molecular identification of some wild Nigerian mushrooms using internal transcribed spacer: polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Phylogenetic Analysis of some Iranian Chrysanthemum morifolium Cultivars, using Internal Transcribed Spacer (ITS) Molecular Markers
Chrysanthemum (Chrysanthemum morifolium) is one of the most important ornamental plants which plays a significant role in the development of gardening industry in the world. The knowledge of genetic diversity is one of the prerequisite criteria for Chrysanthemum breeding with important economic goals. Molecular markers have a significant share in elucidation of inter and intra species genetic d...
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Anopheles (Nyssorhynchus) marajoara is a proven primary vector of malaria parasites in Northeast Brazil, and An. deaneorum is a suspected vector in Western Brazil. Both are members of the morphologically similar Albitarsis Complex, which also includes An. albitarsis and an undescribed species, An. albitarsis "B". These four species were recognized and can be identified using random amplified po...
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Lettuce is belong to the Cichorium family with leaf consumption, Annual cold weather plants and rich in minerals, vitamins and nutrients. The aim of the present study was to evaluate the genetic diversity of 15 varieties and lettuce populations using the ITS area. Sequence quality was evaluated using Chromas 2.1.1 software, aligned by MegAlign 5 software and then similarity matrix and dendrogra...
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A molecular protocol using a hemi-nested polymerase chain reaction (PCR) of the internal transcribed spacer region 2 (ITS2) is reported for the diagnosis of light brown apple moth, Epiphyas postvittana (Walker) (Lepidoptera: Tortricidae), in California. This protocol distinguishes the light brown apple moth from other moths in California based on size differences of PCR amplicons that are visua...
متن کاملPotential bias of fungal 18S rDNA and internal transcribed spacer polymerase chain reaction primers for estimating fungal biodiversity in soil.
Four fungal 18S rDNA and internal transcribed spacer (ITS) polymerase chain reaction (PCR) primer pairs were tested for their specificity towards target fungal DNA in soil DNA extracts, and their ability to assess the diversity of fungal communities in a natural grassland soil was compared. Amplified PCR products were cloned, and approximately 50 clones from each library were sequenced. Phyloge...
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ژورنال
عنوان ژورنال: AMB Express
سال: 2018
ISSN: 2191-0855
DOI: 10.1186/s13568-018-0661-9